Contact Information

Name of PI:
Requested by:
Phone number:
E-mail:
Department:
Shipping *:	Yes No
Delivery Address

* Note: If shipping is not selected, preparation will be stored at -20°C for pick-up. Vector Core location: Academic Research Building (ARB) - Room RG-171.

Billing Information
(orders without PO# will not be processed)

Grant # or PO #:
ICBR # (if applicable)

Vector Preparation

Serotype:	AAV-1 AAV-2 AAV-5 AAV-8 AAV-9 Other Other:
Production Scale Requested: (number of Cell Factories)	CF
If more than one CF, do you want the multiple preps pooled?	Yes No Final volume requested: mL
Vector formulation:	Lactated Ringer's Solution PBS PBS + Ca2+/Mg2+ Other Other:
Does the vector pose any special handling considerations (e.g. oncogenic or toxic transgene product)?	Yes No
If yes, describe the hazard and handling precautions:

Incoming rAAV DNA description

PLEASE SUBMIT (at least) 900 µg of plasmid per cell factory.

Plasmid Name (limited to 12 characters)
Plasmid Lot #
Method of purification (see recommended methods by VC)	CsCI Qiagen Other Other:
Plasmid concentration (by OD260)	µg / µL
Buffer:	TE H2O Other Other:
260/280 ratio:
Volume delivered:	mL
Quality control: Attach gel photo with plasmid	(1) undigested (2) Sma I (3) linearized (4) Other: Click here to send gel picture

Please provide MAPs with restriction sites of interest, or submit DNA sequence.

Does rAAV plasmid DNA contain:	CMV promoter CBA promoter No
If NO is answered please provide at least 200 ng of DNA fragment for Dot Blot analysis. Fragment name:

Are you providing helper plasmid? Yes No

For any questions or concerns, contact the Vector Core team:

Mark Potter and Nathalie Clément

Vector Core Laboratory
Academic Research Building
Rooms RG181-179

Telephone: (352) 273-8900, 273-5522

Email: peds-vcl-orders@peds.ufl.edu